Harmonization of different diffusion MRI images

Hello, I’m considering a dataset containing diffusion-weighted images obtained through dMRI. I’m reconstructing tractography from these images with the scope of analyzing their structural connectivity. I’ve noticed a heterogeneity in the volumes of these images, varying from 30 volumes to even 55. How should I tackle this difference? Does this factor have an impact on the tractography obtainable from an image?

Kind Regards,
Luca