Question About First Level Analysis in AFNI and fMRIPrep Preprocessing

Hi @Steven,
Thank you very much for your reply and for all your ongoing support! Regarding your mention that fMRIPrep includes some skull above, I have a question related to my fMRIPrep output. I noticed that in the EPI-to-anatomical registration, both imaging outputs are not skull stripped. I don’t quite understand why, as I thought fMRIPrep automatically performs skull stripping, but it seems that my images did not undergo this process (see the image below).


However, when I checked the anatomical-to-template alignment, it appears to be quite good, so I’m unsure where the issue may lie.

About your comment regarding the GLM results looking like noise, are you referring to the areas of brain activation in the image appearing chaotic and lacking significant clusters? The reason for this is that I haven’t adjusted the p-value to 0.001 and performed clustering yet. If I implement these settings, it results in no brain activation areas for this subject, making it difficult for me to showcase the brain activation images and demonstrate that my template is not perfectly aligned.

Thank you again for your assistance!

Best regards,
Shuning