Running ANOVA with DTI data in FSL


I am currently running DTI analysis in FSL with 4 groups, and want to run an ANOVA to see if there is a significant effect before I run the post-hoc t-tests. None of the documentation I have found so far applies to this kind of data (FSL has documentation on running an ANOVA on 1 factor with 4 levels but this is not a repeated measures design), so I wanted to see if anyone had advice, or knew if this was possible.

So far my best guess is to set up the design.con and .mat files for a t-test and then manually add 2 columns, but I am not sure if this is good practice.

Any advice would be greatly appreciated!

Thank you.