Hello,

At risk of posting too elementary of question… I’m having trouble with what I should use as a contrast matrix for the following design matrix: seed activity (“beta” - column 1); two experimental conditions (“ISOLD” & “ISNEW” - columns 2/3 in the following; and an intercept variable (column 4).

If I’m interested in **seed connectivity in ISNEW > seed connectivity in ISOLD**, what would my contrast matrix look like? [0 -1 1 0] would not take into account the seed connectivity, correct?

Thanks,

Tom